Article
Detection of Lassa virus antinucleoprotein immunoglobulin G (IgG) and IgM antibodies by a simple recombinant immunoblot assay for field use.
Journal of clinical microbiology - 1 Nov 1998
Ter Meulen J, Koulemou K, Wittekindt T, Windisch K, Strigl S, Conde S, Schmitz H
Abstract excerpt
The nucleoprotein of Lassa virus, strain Josiah, was expressed in Escherichia coli as an N-terminally truncated, histidine-tagged recombinant protein. Following affinity purification the protein was completely denatured and spotted onto nitrocellulose membrane. A total of 1 microgram of protein w...
Topics
- Amino Acid Sequence
- Antibodies, Viral
- Base Sequence
- Capsid
- Capsid Proteins
- DNA Primers
- DNA, Viral
- Diagnostic Errors
- Escherichia coli
- Fluorescent Antibody Technique, Indirect
- Genetic Variation
- Germany
- Guinea
- Humans
- Immunoblotting