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Article

PCR amplification and characterization of the intergenic spacer region of the ribosomal DNA in Pyrenophora graminea.

FEMS microbiology letters - 1 Sept 1998

Pecchia S, Mercatelli E, Vannacci G

Abstract excerpt

Successful amplification of the whole intergenic spacer region of the nuclear ribosomal repeat (IGS) in Pyrenophora graminea was obtained with a PCR-based assay. Single amplification products showed length differences. Depending on the length of the IGS-PCR product, ca. 3.8 or 4.4 kb, two groups of isolates could be identified. The RFLP patterns of isolates obtained with the 6-base cutting enzymes Apal, BglII,...

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Identifiers

PMID: 9741081
DOI: 10.1111/j.1574-6968.1998.tb13178.x

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