PCR amplification and characterization of the intergenic spacer region of the ribosomal DNA in Pyrenophora graminea.

doi:10.1111/j.1574-6968.1998.tb13178.x

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Article

PCR amplification and characterization of the intergenic spacer region of the ribosomal DNA in Pyrenophora graminea.

FEMS microbiology letters - 1 Sept 1998

Pecchia S, Mercatelli E, Vannacci G

Abstract excerpt

Successful amplification of the whole intergenic spacer region of the nuclear ribosomal repeat (IGS) in Pyrenophora graminea was obtained with a PCR-based assay. Single amplification products showed length differences. Depending on the length of the IGS-PCR product, ca. 3.8 or 4.4 kb, two groups...

Topics

Ascomycota
Base Sequence
DNA Primers
DNA, Fungal
DNA, Ribosomal
Genetic Variation
Hordeum
Plant Diseases
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Restriction Mapping
Temperature

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