A Nested-PCR-Based Schizodeme Method for Identifying <i>Leishmania</i> Kinetoplast Minicircle Classes Directly from Clinical Samples and Its Application to the Study of the Epidemiology of <i>Leishmania tropica</i> in Pakistan

Abstract excerpt

A nested PCR was developed to amplify the variable region of the kinetoplast minicircles of all Leishmania species which infect mammals. Each Leishmania parasite contains approximately 10,000 kinetoplast DNA minicircles, which are unequally distributed among approximately 10 minicircle classes. The PCR primers were designed to bind within the 120-bp conserved region which is common to all minicircle classes; the...

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