Article
Genotyping of apolipoprotein E by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Rapid communications in mass spectrometry : RCM - 1 Jan 1998
Srinivasan J R, Kachman M T, Killeen A A, Akel N, Siemieniak D, Lubman D M
Abstract excerpt
The genotyping of the various isoforms of Apolipoprotein E (apo E) has been performed using matrix-assisted laser desorption/ionization (MALDI-MS). The polymerase chain reaction was used to amplify the specific apo E gene sequence followed by digestion with Cfo I (Clostridium formicoaceticum), fo...
Topics
- Apolipoproteins E
- Base Sequence
- DNA
- Exonucleases
- Genotype
- Humans
- Hydrolysis
- Molecular Sequence Data
- Polymerase Chain Reaction
- Restriction Mapping
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization