Optimization of PCR and electrophoresis conditions enhances mutation analysis of the BRCA1 gene.

doi:10.1006/mgme.1998.2713

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Article

Optimization of PCR and electrophoresis conditions enhances mutation analysis of the BRCA1 gene.

Molecular genetics and metabolism - 1 Jul 1998

Lallas T A, Buller R E

Abstract excerpt

Single-strand confirmation polymorphism (SSCP) analysis on MDE gels has been extensively used to detect BRCA1 gene mutations. In screening a large cohort of ovarian cancer patients, our group was not completely satisfied with the standard published techniques. Although conventional primer sets us...

Topics

Buffers
DNA Mutational Analysis
DNA Primers
Electrophoresis
Exons
Female
Genes, BRCA1
Humans
Mutation
Ovarian Neoplasms
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Temperature
Time Factors

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