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Article

Optimization of PCR and electrophoresis conditions enhances mutation analysis of the BRCA1 gene.

Molecular genetics and metabolism - 1 Jul 1998

Lallas T A, Buller R E

Abstract excerpt

Single-strand confirmation polymorphism (SSCP) analysis on MDE gels has been extensively used to detect BRCA1 gene mutations. In screening a large cohort of ovarian cancer patients, our group was not completely satisfied with the standard published techniques. Although conventional primer sets usually amplified well, we were able to enhance band spread and resolution by varying the PCR and electrophoresis...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 9719625
DOI: 10.1006/mgme.1998.2713

Genes

BRCA1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Optimization of PCR and electrophoresis conditions enhances mutation analysis of the BRCA1 gene.PMID 9719625Read ↗

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