Effect of truncation and mutation of the carboxyl-terminal region of the beta subunit on membrane assembly and activity of the pyridine nucleotide transhydrogenase of Escherichia coli.

Abstract excerpt

The pyridine nucleotide transhydrogenase of Escherichia coli is a proton pump composed of two different subunits (alpha and beta) assembled as a tetramer (alpha 2 beta 2) in the cytoplasmic membrane. A series of mutants was generated in which the carboxyl-terminal region of the beta subunit was progressively truncated. Removal of the two carboxyl-terminal amino acid residues prevented incorporation of the enzyme...

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