Article
Generation of up-regulated allosteric variants of potato ADP-glucose pyrophosphorylase by reversion genetics.
Proceedings of the National Academy of Sciences of the United States of America - 18 Aug 1998
Greene T W, Kavakli I H, Kahn M L, Okita T W
Abstract excerpt
Mutagenesis of the large subunit (LS) of the potato ADP-glucose pyrophosphorylase generated an enzyme, P52L, that was insensitive to 3-phosphoglycerate (3-PGA). To identify additional residues involved in 3-PGA interaction, we subjected P52L LS DNA to a second round of mutagenesis and identified...
Topics
- Allosteric Site
- Amino Acid Sequence
- Base Sequence
- DNA Primers
- Enzyme Activation
- Enzyme Stability
- Genes, Plant
- Genetic Variation
- Glucose-1-Phosphate Adenylyltransferase
- Glyceric Acids
- Hot Temperature
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed