Overexpression of lactate dehydrogenase A attenuates glucose‐induced insulin secretion in stable MIN‐6 β‐cell lines

Abstract excerpt

Since islet beta-cells express little L-lactate dehydrogenase (LDH) activity, we have examined the effects on these cells of LDH overexpression. In mock-transfected MIN6 beta-cells, LDH activity was 38 nmol/min/mg protein, and 30 mM glucose stimulated secretion 10.4-fold. In two MIN6 cell clones stably overexpressing human LDH-A cDNA, insulin secretion was stimulated only 2.7- and 2.1-fold by high glucose....

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