Article
Three rhamnosyltransferases responsible for assembly of the A-band D-rhamnan polysaccharide in Pseudomonas aeruginosa: a fourth transferase, WbpL, is required for the initiation of both A-band and B-band lipopolysaccharide synthesis.
Molecular microbiology - 1 Jun 1998
Rocchetta H L, Burrows L L, Pacan J C, Lam J S
Abstract excerpt
The Pseudomonas aeruginosa A-band lipopolysaccharide (LPS) molecule has an O-polysaccharide region composed of trisaccharide repeat units of alpha1-->2, alpha1-->3, alpha1-->3 linked D-rhamnose (Rha). The A-band polysaccharide is assembled by the alpha-D-rhamnosyltransferases, WbpX, WbpY and WbpZ. WbpZ probably transfers the first Rha residue onto the A-band accepting molecule, while WbpY and WbpX subsequently...
Topics
- Adenosine Triphosphatases
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Carbohydrate Sequence
- Cloning, Molecular
- DNA Helicases
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Escherichia coli Proteins