Evaluation of a spectrophotometric assay for the measurement of malondialdehyde and 4‐hydroxyalkenals in human spermatozoa: relationships with semen quality and sperm function

Abstract excerpt

A spectrophotometric assay for the measurement of malondialdehyde and 4 hydroxyalkenals (MA + 4HA) has been evaluated for the detection of sperm pathologies involving oxidative stress. In order to make sensitive measurements of MA + 4HA on human spermatozoa, the stimulation of a lipid peroxidation cascade with a ferrous ion promoter was found to be necessary. The optimal configuration for the promoter was defined...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Pesticide Exposure and Toxicity
• Share to Reproductive Biology and Fertility
• Share to Sperm and Testicular Function