Expression of a Gene for a Porin-Like Protein of the OmpA Family fromMycobacterium tuberculosisH37Rv

Abstract excerpt

An open reading frame in the genomic database of Mycobacterium tuberculosis H37Rv was identified as having homology with an outer membrane protein. We found that the gene specified a protein belonging to the OmpA family, which includes some porins of gram-negative organisms. The gene was amplified by PCR and cloned into Escherichia coli. Overexpression of the gene was toxic to the host, but limited amounts could...

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