Article
Genotyping of bovine kappa-casein (kappa-CNA, kappa-CNB, kappa-CNC, kappa-CNE) following DNA sequence amplification and direct sequencing of kappa-CNE PCR product.
Animal genetics - 1 Jan 1991
Schlieben S, Erhardt G, Senft B
Abstract excerpt
Genomic DNA isolated from blood and semen of dairy cattle with known kappa-casein (kappa-CN) genotypes was subjected to Southern blot hybridization and polymerase chain reaction (PCR) using up to 14 restriction endonucleases. kappa-casein genotypes AA, AB and BB were identified using Hin dIII and...
Topics
- Animals
- Base Sequence
- Blotting, Southern
- Caseins
- Cattle
- DNA Restriction Enzymes
- Exons
- Genotype
- Introns
- Molecular Sequence Data
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
