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New PCR Assay Using Glucose-6-Phosphate Dehydrogenase for Identification of <i>Leishmania</i> Species

1 Feb 2003

Abstract excerpt

Glucose-6-phosphate dehydrogenase (G6PD) is one of the multilocus enzymes used to identify Leishmania by zymodeme analysis. The polymorphic pattern revealed by partial characterization of the gene encoding G6PD generated molecular markers useful in the identification of different Leishmania species by PCR. Initially degenerate oligonucleotides were designed on the basis of data on the conserved active center...

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PMID: 12574243
DOI: 10.1128/jcm.41.2.540-546.2003

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