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Article

Simplified polymerase chain reaction detection of new world Leishmania in clinical specimens of cutaneous leishmaniasis.

1 Jan 1998

Abstract excerpt

Polymerase chain reaction (PCR)-based detection of New World Leishmania from different types of clinical specimens has been further streamlined for field use by simplifying sample preparation and modifying published protocols. A multiplex PCR reaction was developed that allows simultaneous detection of the Leishmania genus and identification of the L. braziliensis complex. For typing isolates in culture, we found...

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PMID: 9452300
DOI: 10.4269/ajtmh.1998.58.102

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