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Article

Quantitative trait locus mapping in dairy cattle by means of selective milk DNA pooling using dinucleotide microsatellite markers: analysis of milk protein percentage.

Genetics - 1 Jul 1998

Lipkin E, Mosig M O, Darvasi A, Ezra E, Shalom A, Friedmann A, Soller M

Abstract excerpt

"Selective DNA pooling" accomplishes quantitative trait locus (QTL) mapping through densitometric estimates of marker allele frequencies in pooled DNA samples of phenotypically extreme individuals. With poly(TG) microsatellites, such estimates are confounded by "shadow" ("stutter") bands. A correction procedure was developed on the basis of an observed linear regression between shadow band intensity and allele TG...

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Identifiers

PMID: 9649542
DOI: 10.1093/genetics/149.3.1557
PMCID: PMC1460242

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