Article
Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region.
Nature biotechnology - 1 Dec 1996
Ueda H, Tsumoto K, Kubota K, Suzuki E, Nagamune T, Nishimura H, Schueler P A, Winter G, Kumagai I, Mohoney W C
Abstract excerpt
We describe an immunoassay that is based on the interchain interaction of separated VL and VH chains from a single chain antibody variable region. In the presence of antigen, the chains reassociate. VL fragments of anti-hen egg lysozyme (HEL) antibody HyHEL-10 were immobilized on microtiter plates. Samples were coincubated with an M13-displayed VH chain, and assayed with peroxidase-labeled anti-M13 antibody....
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