Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region.

doi:10.1038/nbt1296-1714

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Article

Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region.

Nature biotechnology - 1 Dec 1996

Ueda H, Tsumoto K, Kubota K, Suzuki E, Nagamune T, Nishimura H, Schueler P A, Winter G, Kumagai I, Mohoney W C

Abstract excerpt

We describe an immunoassay that is based on the interchain interaction of separated VL and VH chains from a single chain antibody variable region. In the presence of antigen, the chains reassociate. VL fragments of anti-hen egg lysozyme (HEL) antibody HyHEL-10 were immobilized on microtiter plate...

Topics

Animals
Antigen-Antibody Reactions
Bacteriophages
Biosensing Techniques
Chickens
Enzyme-Linked Immunosorbent Assay
Immunoglobulin Fragments
Immunoglobulin Variable Region
Muramidase
Mutation

Source

Open in PubMed

Identifiers

PMID: 9634858
DOI: 10.1038/nbt1296-1714

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9634858-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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