Article
Construction of PCR-ligated long flanking homology cassettes for use in the functional analysis of six unknown open reading frames from the left and right arms of Saccharomyces cerevisiae chromosome XV.
Yeast (Chichester, England) - 15 Mar 1998
Pearson B M, Hernando Y, Schweizer M
Abstract excerpt
Six open reading frames (ORFs) of unknown function from Saccharomyces cerevisiae chromosome XV, three from the left and three from the right arm, were deleted in two diploid strains by the short flanking homology method (Wach et al., 1994). Transformants were selected as Geneticin (G418)-resistant colonies and correct integration of the kanMX4 cassette was checked by colony PCR. Following sporulation of the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.