Article
TMIG-2DPAGE: a new concept of two-dimensional gel protein database for research on aging.
Electrophoresis - 1 Feb 1998
Toda T, Kaji K, Kimura N
Abstract excerpt
Cellular proteins of a normal human diploid fibroblast line (TIG-3) at various stages of replicative aging were resolved by horizontal isoelectric focusing on an immobilized pH gradient, followed by vertical sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). Spot proteins were visualized by silver staining and quantitated by image processing. All corresponding spots were matched among...
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