Oxidation-reduction properties of the regulatory site of spinach phosphoribulokinase.

doi:10.1006/abbi.1997.0499

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Article

Oxidation-reduction properties of the regulatory site of spinach phosphoribulokinase.

Archives of biochemistry and biophysics - 1 Feb 1998

Hirasawa M, Brandes H K, Hartman F C, Knaff D B

Abstract excerpt

The oxidation-reduction midpoint potential (Em) of the regulatory disulfide, formed between Cys16 and Cys55, of spinach chloroplast phosphoribulokinase has been determined both for the wild-type enzyme and for a C244S-C250S double mutant, using enzymatic activity to monitor the oxidation-reductio...

Topics

Chloroplasts
Cysteine
Disulfides
Enzyme Activation
Hydrogen-Ion Concentration
Mutation
Oxidation-Reduction
Phosphotransferases (Alcohol Group Acceptor)
Potentiometry
Spinacia oleracea

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