Article
The involvement of the two acidic patches of spinach plastocyanin in the reaction with photosystem I.
Biochimica et biophysica acta - 15 Dec 1997
Young S, Sigfridsson K, Olesen K, Hansson O
Abstract excerpt
Six different spinach plastocyanin mutants have been constructed by site-directed mutagenesis and expressed in Escherichia coli to probe the importance of the two acidic patches in the interaction with photosystem I. The mutants were: Asp42Lys, Glu43Asn, Glu43Lys, Glu43Gln/Asp44Asn, Glu59Lys/Glu60Gln and Glu43Asn/Glu59Lys/Glu60Gln and they have been characterised by optical absorption and EPR spectroscopy, redox...
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