Article
The involvement of the two acidic patches of spinach plastocyanin in the reaction with photosystem I.
Biochimica et biophysica acta - 15 Dec 1997
Young S, Sigfridsson K, Olesen K, Hansson O
Abstract excerpt
Six different spinach plastocyanin mutants have been constructed by site-directed mutagenesis and expressed in Escherichia coli to probe the importance of the two acidic patches in the interaction with photosystem I. The mutants were: Asp42Lys, Glu43Asn, Glu43Lys, Glu43Gln/Asp44Asn, Glu59Lys/Glu6...
Topics
- Electron Transport
- Escherichia coli
- Isoelectric Point
- Kinetics
- Models, Chemical
- Mutagenesis, Site-Directed
- Mutation
- Photolysis
- Photosynthetic Reaction Center Complex Proteins
- Photosystem I Protein Complex
- Plastocyanin
- Recombinant Fusion Proteins
- Spinacia oleracea