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Article

Detection of Mycoplasma hyopneumoniae by Air Sampling with a Nested PCR Assay

1 Feb 1998

Abstract excerpt

This article describes the first successful detection of airborne Mycoplasma hyopneumoniae under experimental and field conditions with a new nested PCR assay. Air was sampled with polyethersulfone membranes (pore size, 0.2 micron) mounted in filter holders. Filters were processed by dissolution and direct extraction of DNA for PCR analysis. For the PCR, two nested pairs of oligonucleotide primers were designed...

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Identifiers

PMID: 9464391
DOI: 10.1128/aem.64.2.543-548.1998

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