Article
The diversity of Mycoplasma hyopneumoniae within and between herds using pulsed-field gel electrophoresis.
Veterinary microbiology - 10 Aug 2005
Stakenborg Tim, Vicca Jo, Butaye Patrick, Maes Dominiek, Peeters Johan, de Kruif Aart, Haesebrouck Freddy
Abstract excerpt
Over the years, pulsed-field gel electrophoresis (PFGE) has been proven a robust technique to type isolates with a high resolution and a good reproducibility. In this study, a PFGE protocol is described for the typing of Mycoplasma hyopneumoniae isolates. The potential of this technique was demonstrated by comparing M. hyopneumoniae isolates obtained from the same as well as from different herds. The use of two...
Topics
- Animals
- Cluster Analysis
- DNA, Bacterial
- Deoxyribonucleases, Type II Site-Specific
- Electrophoresis, Gel, Pulsed-Field
- Europe
- Genetic Variation
- Molecular Epidemiology
- Mycoplasma hyopneumoniae
- Pneumonia of Swine, Mycoplasmal