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Article

A poplar plastocyanin mutant suitable for adsorption onto gold surface via disulfide bridge.

Archives of biochemistry and biophysics - 1 Mar 2002

Andolfi L, Cannistraro S, Canters G W, Facci P, Ficca A G, Van Amsterdam I M C, Verbeet M Ph

Abstract excerpt

Aiming to achieve stable immobilization for a redox-active cupredoxin protein onto a gold substrate and its consequent molecular level monitoring by Scanning Tunnelling Microscopy (STM), we introduced a disulphide bridge within poplar plastocyanin, while avoiding the perturbation of its active site. We selected and modified residues Ile-21 to Cys and Glu-25 to Cys by structurally conservative mutagenesis. Optical...

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PMID: 11883906
DOI: 10.1006/abbi.2001.2735

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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