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Article

Microscopic pKa values of Escherichia coli thioredoxin.

Biochemistry - 2 Dec 1997

Chivers P T, Prehoda K E, Volkman B F, Kim B M, Markley J L, Raines R T

Abstract excerpt

Thiol:disulfide oxidoreductases have a CXXC motif within their active sites. To initiate the reduction of a substrate disulfide bond, the thiolate form of the N-terminal cysteine residue (CXXC) of this motif performs a nucleophilic attack. Escherichia coli thioredoxin [Trx (CGPC)] is the best characterized thiol:disulfide oxidoreductase. Previous determinations of the active-site pKa values of Trx have led to...

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PMID: 9398223
DOI: 10.1021/bi970071j

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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