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Article

A gene (wbbL) from Serratia marcescens N28b (O4) complements the rfb-50 mutation of Escherichia coli K-12 derivatives.

Journal of bacteriology - 1 Dec 1997

Rubirés X, Saigi F, Piqué N, Climent N, Merino S, Albertí S, Tomás J M, Regué M

Abstract excerpt

A cosmid-based genomic library of Serratia marcescens N28b was introduced into Escherichia coli DH5alpha, and clones were screened for serum resistance. One clone was found resistant to serum, to bacteriocin 28b, and to bacteriophages TuIa and TuIb. This clone also showed O antigen in its lipopolysaccharide. Subcloning and sequencing experiments showed that a 2,124-bp DNA fragment containing the rmlD and wbbL...

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Identifiers

PMID: 9393727
DOI: 10.1128/jb.179.23.7581-7586.1997
PMCID: PMC179713

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