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Amplification of Avian Reovirus RNA Using the Reverse Transcriptase-Polymerase Chain Reaction

1 Jul 1997

Abstract excerpt

A reverse transcriptase-polymerase chain reaction method was developed for the detection of avian reovirus. The origin of primers was from the S1 gene of the avian reovirus genome. A reovirus-specific 532-base pair cDNA product was amplified by these primers from six reference strains and 23 field isolates of avian reoviruses, but not from seven different avian pathogenic viruses and bacteria. As little as 1 pg...

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PMID: 9356712
DOI: 10.2307/1592157

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