Article
Detection of mammalian reovirus RNA by using reverse transcription-PCR: sequence diversity within the lambda3-encoding L1 gene.
Journal of clinical microbiology - 1 Apr 2002
Leary Thomas P, Erker James C, Chalmers Michelle L, Cruz Andrea T, Wetzel J Denise, Desai Suresh M, Mushahwar Isa K, Dermody Terence S
Abstract excerpt
Reoviruses infect virtually all mammalian species, and infection of humans is associated with mild gastrointestinal or upper respiratory illnesses. To improve reovirus detection strategies, we developed a reverse transcription-PCR technique to amplify a fragment of the reovirus L1 gene segment. This assay was capable of detecting 44 of 44 reovirus field isolate strains and was sufficiently sensitive to detect...
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