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A Multiplex RT-PCR for the Detection of Astrovirus, Rotavirus, and Reovirus in Turkeys

1 Sept 2012

Abstract excerpt

This study was undertaken to develop and validate a multiplex reverse transcription-polymerase chain reaction (mRT-PCR) for simultaneous detection of avian rotavirus, turkey astrovirus-2 (TAstV-2), and avian reovirus. Primers targeting the conserved regions of NSP4 gene of avian rotavirus, polymerase gene of TAstV-2, and S4 gene of avian reovirus were used. The position of bands at 630, 802, and 1120 base pairs...

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PMID: 23050480
DOI: 10.1637/9958-100911-resnote.1

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