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Detection of flaviviruses by reverse‐transcriptase polymerase chain reaction

1 Apr 1991

Abstract excerpt

RNA sequences of five flaviviruses were detected by a modified polymerase chain reaction (PCR) that incorporated a reverse transcriptase and RNase inhibitor. Oligonucleotide primer pairs were synthesized to amplify sequences from St. Louis encephalitis (SLE), Japanese encephalitis (JBE), yellow fever (YF), dengue 2 (DEN-2), and dengue 4 (DEN-4) viruses. The amplified products were visualized as bands of...

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PMID: 1713265
DOI: 10.1002/jmv.1890330410

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