Article
Construction of a double hha hns mutant of Escherichia coli: effect on DNA supercoiling and alpha-haemolysin production.
FEMS microbiology letters - 1 Oct 1997
Nieto J M, Mouriño M, Balsalobre C, Madrid C, Prenafeta A, Muñoa F J, Juárez A
Abstract excerpt
A double hha hns Escherichia coli mutant was constructed. The effect of the single hns mutation and of the double hha hns mutation on the expression of the alpha-haemolysin determinant of plasmid pANN202-312 was assessed. Whereas the hns mutant moderately increased expression of the toxin, the double hha hns mutant strongly enhanced transcription of the hly operon and hence expression of the toxin. This suggests...
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