Article
Construction of a double hha hns mutant of Escherichia coli: effect on DNA supercoiling and alpha-haemolysin production.
FEMS microbiology letters - 1 Oct 1997
Nieto J M, Mouriño M, Balsalobre C, Madrid C, Prenafeta A, Muñoa F J, Juárez A
Abstract excerpt
A double hha hns Escherichia coli mutant was constructed. The effect of the single hns mutation and of the double hha hns mutation on the expression of the alpha-haemolysin determinant of plasmid pANN202-312 was assessed. Whereas the hns mutant moderately increased expression of the toxin, the do...
Topics
- Bacterial Proteins
- DNA, Bacterial
- DNA, Superhelical
- DNA-Binding Proteins
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Genes, Reporter
- Hemolysin Proteins
- Mutation
- Nucleic Acid Conformation
- Plasmids