Article
Mutations in B-type natriuretic peptide mediating receptor-A selectivity.
FEBS letters - 8 Sept 1997
Schoenfeld J R, Tom J Y, Lowe D G
Abstract excerpt
Libraries of monovalent display-phage expressing mutant human B-type natriuretic peptide (hBNP) were used to identify variants that preferentially bind natriuretic peptide receptor-A (NPR-A) compared to receptor-C (NPR-C). Position 19 was a significant determinant of receptor specificity for hBNP...
Topics
- Amino Acid Sequence
- Atrial Natriuretic Factor
- Base Sequence
- Binding, Competitive
- Cell Line
- Cyclic GMP
- DNA Primers
- Gene Library
- Genetic Variation
- Guanylate Cyclase
- Humans
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Natriuretic Peptide, Brain
- Polymerase Chain Reaction
- Receptors, Atrial Natriuretic Factor
- Recombinant Proteins