Article
Genotoxicity monitoring of small bodies of water using two species of tadpoles and the alkaline single cell gel (comet) assay.
Environmental and molecular mutagenesis - 1 Jan 1997
Ralph S, Petras M
Abstract excerpt
To monitor genotoxicity in small bodies of water (e.g., creeks, ponds, and drainage ditches) we examined tadpole erythrocytes of two species: Rana clamitans and Rana pipiens,using the alkaline single cell gel DNA electrophoresis (SCG) or "comet" assay. This approach involves detection, under alkaline conditions, of cell DNA fragments which on electrophoresis migrate from the nuclear core, resulting in a "comet...
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