Article
Determination of penicillin susceptibility of Streptococcus pneumoniae using the polymerase chain reaction.
Molecular pathology : MP - 1 Feb 1997
Jalal H, Organji S, Reynolds J, Bennett D, O'Mason E, Millar M R
Abstract excerpt
AIM: To develop a polymerase chain reaction (PCR) based method to detect penicillin susceptibility in isolates of Streptococcus pneumoniae (SP). METHOD: PCR primers were designed to amplify differential nucleotide sequences of the penicillin-binding protein (PBP) genes 2b, 2x, and 1a in penicillin susceptible and resistant strains of SP. Primers derived from the PBP 2x and 2b genes were designed to amplify...
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