Article
Enzymatic assay of beta-lactamase using circular dichroism spectropolarimetry.
Analytical biochemistry - 1 May 1997
Long D M
Abstract excerpt
A method for measuring the rates of enzymatic hydrolysis of beta-lactam antibiotics based on circular dichroism spectropolarimetry is described. Unhydrolyzed beta-lactam antibiotics have high molar ellipticities, but the hydrolyzed compounds are circular dichroism (CD) inactive in the case of penams or have significantly different CD spectra in the case of cephems. By measuring CD at constant wavelength as a...
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