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Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model

1 Apr 1997

Abstract excerpt

A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from...

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Share to Clostridium difficile and Clostridium perfringens research
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PMID: 9157172
DOI: 10.1128/jcm.35.4.995-998.1997

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