Article
Decrease in expression of the master operon of flagellin synthesis in a dnaA46 mutant of Escherichia coli.
Biological & pharmaceutical bulletin - 1 Apr 1997
Mizushima T, Koyanagi R, Katayama T, Miki T, Sekimizu K
Abstract excerpt
We examined the expression of the fliC, fliA and fihD genes in the dnaA46 mutant. In Northern blot analysis, the amounts of fliC, fliA, and flhD mRNA decreased in the dnaA46 mutant growing at 37 degrees C but not at 28 degrees C. The promoter activity of each gene also decreased in this mutant at 37 degrees C. The decrease in expression of the flh D gene was not related to the cAMP-catabolite activator protein...
Topics
- Blotting, Northern
- Cyclic AMP
- Cyclic AMP Receptor Protein
- Escherichia coli
- Flagellin
- Gene Expression Regulation, Bacterial
- Mutation
- Operon
- RNA, Messenger