Article
Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53.
Virology - 14 Apr 1997
Kinney R M, Butrapet S, Chang G J, Tsuchiya K R, Roehrig J T, Bhamarapravati N, Gubler D J
Abstract excerpt
We identified nine nucleotide differences between the genomes of dengue-2 (DEN-2) 16681 virus and its vaccine derivative, strain PDK-53. These included a C-to-T (16681-to-PDK-53) mutation at nucleotide position 57 of the 5'-untranslated region, three silent mutations, and substitutions prM-29 Asp...
Topics
- Animals
- Base Sequence
- Cell Line
- DNA, Complementary
- Dengue
- Dengue Virus
- Disease Models, Animal
- Genome, Viral
- Macaca mulatta
- Mice
- Mice, Inbred ICR
- Molecular Sequence Data
- Phenotype
- Sequence Analysis, DNA
- Species Specificity
- Temperature
- Vaccines, Attenuated
- Viral Envelope Proteins