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Detection of Flaviviruses by Reverse Transcriptase‐Polymerase Chain Reaction with the Universal Primer Set

1 Mar 1997

Abstract excerpt

Using a universal primer set designed to match the sequence of the NS1 gene of flaviviruses, the virus RNA of dengue (DEN), Japanese encephalitis (JEV), powassan and langat of Flaviviridae were successfully amplified by polymerase chain reaction (PCR) via cDNA; and with different internal primers, the serotypes of the dengue viruses were identified. Of the 78 clinically diagnosed dengue fever patients, 18...

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PMID: 9130232
DOI: 10.1111/j.1348-0421.1997.tb01192.x

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