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TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus.

1 Mar 2018

Abstract excerpt

OBJECTIVE: To detect Japanese encephalitis virus (JEV) rapidly and distinguish its genotypes, a TaqMan-based reverse transcriptase quantitative polymerase chain reaction (RT-PCR) detection system was developed. METHODS: By aligning the full-length sequences of JEV (G1-G5), six sets of highly specific TaqMan real-time RT-PCR primers and probes were designed based on the highly conserved NS1, NS2, and M genes of...

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PMID: 29673443
DOI: 10.3967/bes2018.026

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