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Fast Duplex One-Step Reverse Transcriptase PCR for Rapid Differential Detection of West Nile and Japanese Encephalitis Viruses

16 Sept 2010

Abstract excerpt

The aim of this study was to develop a highly sensitive and specific one-step duplex reverse transcriptase PCR (RT-PCR) assay for the simultaneous and differential detection of West Nile (WNV) and Japanese encephalitis (JEV) viruses. The bioinformatic analysis of published sequences of WNV and JEV revealed conserved regions not targeted by previously reported primers. A total of 13 primers were designed based on...

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PMID: 20844215
DOI: 10.1128/jcm.00582-10

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