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Article

Characterization of recombinant soybean leghemoglobin a and apolar distal histidine mutants.

Journal of molecular biology - 14 Mar 1997

Hargrove M S, Barry J K, Brucker E A, Berry M B, Phillips G N, Olson J S, Arredondo-Peter R, Dean J M, Klucas R V, Sarath G

Abstract excerpt

The cDNA for soybean leghemoglobin a (Lba) was cloned from a root nodule cDNA library and expressed in Escherichia coli. The crystal structure of the ferric acetate complex of recombinant wild-type Lba was determined at a resolution of 2.2 A. Rate constants for O2, CO and NO binding to recombinant Lba are identical with those of native soybean Lba. Rate constants for hemin dissociation and auto-oxidation of...

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PMID: 9086279
DOI: 10.1006/jmbi.1996.0833

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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