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An optimized PCR leads to rapid and highly sensitive detection of Borrelia burgdorferi in patients with Lyme borreliosis

1 Mar 1997

Abstract excerpt

The present study aimed at developing an optimized PCR protocol fro the sensitive and specific detection of all three Borrelia burgdorferi genospecies pathogenic to humans in Lyme borreliosis patients. A rapid DNA extraction method using alkaline lysis was introduced and was found to be superior to other DNA extraction methods. Nested PCR was performed with primer sets targeting the plasmid-located ospA gene and...

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Identifiers

PMID: 9041413
DOI: 10.1128/jcm.35.3.685-690.1997

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Source receipt: pubmed, retrieved 7 Sept 2026.

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