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Article

Distribution of Clinically RelevantBorreliaGenospecies in Ticks Assessed by a Novel, Single-Run, Real-Time PCR

1 Jan 2002

Abstract excerpt

A LightCycler-based PCR protocol was developed which targets the ospA gene for the identification and quantification of the different Borrelia burgdorferi sensu lato species in culture and in ticks, based on the use of a fluorescently labeled probe (HybProbe) and an internally labeled primer. The detection limit of the PCR was 1 to 10 spirochetes. A melting temperature determined from the melting curve of the...

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PMID: 11773090
DOI: 10.1128/jcm.40.1.36-43.2002

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