Article
L-cysteine biosynthesis in Bacillus subtilis: identification, sequencing, and functional characterization of the gene coding for phosphoadenylylsulfate sulfotransferase.
Journal of bacteriology - 1 Feb 1997
Mansilla M C, de Mendoza D
Abstract excerpt
Random Tn917 mutagenesis of Bacillus subtilis followed by selection of lipoic acid auxotrophs led to the isolation of the cysH gene. The gene was sequenced and found to encode a phosphoadenylylsulfate sulfotransferase with a molecular mass of 27 kDa. Expression of lacZ fused to the cysH promoter...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- Cysteine
- Enzyme Repression
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Recombinant Proteins
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Sulfides
- Sulfotransferases