Article
Display of beta-lactamase on the Escherichia coli surface: outer membrane phenotypes conferred by Lpp'-OmpA'-beta-lactamase fusions.
Protein engineering - 1 Feb 1996
Georgiou G, Stephens D L, Stathopoulos C, Poetschke H L, Mendenhall J, Earhart C F
Abstract excerpt
Bacterial cell-surface exposure of foreign peptides and soluble proteins has been achieved recently by employing a fusion protein methodology. An Lpp'-OmpA(46-159)-Bla fusion protein has been shown previously to display the normally periplasmic enzyme beta-lactamase (Bla) on the cell surface of t...
Topics
- Amino Acid Sequence
- Anti-Bacterial Agents
- Bacterial Outer Membrane Proteins
- Blotting, Western
- Carrier Proteins
- Cell Wall
- Edetic Acid
- Electrophoresis, Polyacrylamide Gel
- Epitopes
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression
- Immunohistochemistry
- Lipoproteins
- Membrane Proteins
- Microscopy, Immunoelectron
- Molecular Sequence Data
- Permeability