Article
STR typing of buccal swabs for paternity testing with reference to Japanese population data on the D20S85, D14S118, and D14S543 loci.
Nihon hoigaku zasshi = The Japanese journal of legal medicine - 1 Dec 1996
Okazaki K, Kishida T, Wang W, Nakamura M, Tamaki Y
Abstract excerpt
To simplify short tandem repeat (STR) typing of genomic DNA, we used buccal cells instead of blood cells. Buccal swabs taken from paternity-test trios were washed with 1 ml of distilled water by centrifugation, and the pellets were suspended in 20 microliters of distilled water. The suspensions were frozen at -80 degrees C and then thawed. Using a 1/5 volume of each suspension, we were able to type STRs at the...
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