Article
RP4::Mu3A-mediated in vivo cloning and transfer of a chlorobiphenyl catabolic pathway.
Microbiology (Reading, England) - 1 Nov 1996
Springael D, van Thor J, Goorissen H, Ryngaert A, De Baere R, Van Hauwe P, Commandeur L C, Parsons J R, De Wachter R, Mergeay M
Abstract excerpt
Chromosomal DNA fragments encoding the ability to utilize biphenyl as sole carbon source (Bph+) were mobilized by means of plasmid RP4::Mu3A from strain JB1 (tentatively identified as Burkholderia sp.) to Alcaligenes eutrophus CH34 at a frequency of 10(-3) per transferred plasmid. The mobilized DNA integrated into the recipient chromosome or was recovered as catabolic prime plasmids. Three Bph+ prime plasmids...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.