Article
Application of long PCR method of identification of variations in nucleotide sequences among varicella-zoster virus isolates.
Journal of clinical microbiology - 1 Dec 1996
Takayama M, Takayama N, Inoue N, Kameoka Y
Abstract excerpt
Restriction fragment length polymorphism (RFLP) analysis of whole viral DNA of varicella-zoster virus (VZV) requires the time-consuming and laborious preparation of a large amount of purified viral DNA. RFLP analysis of small DNA fragments amplified by PCR was developed as an alternative method. However, its use has been limited because of the small number of variations in VZV. To overcome these drawbacks and to...
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