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Genotyping hepatitis C virus isolates from Spain, Brazil, China, and Macau by a simplified PCR method.

Journal of clinical microbiology - 1 Oct 1996

Holland P V, Barrera J M, Ercilla M G, Yoshida C F, Wang Y, de Olim G A, Betlach B, Kuramoto K, Okamoto H

Abstract excerpt

An improved and simplified method of genotyping was developed for classifying hepatitis C virus (HCV) isolates into the five common genotypes, i.e., I/1a, II/1b, III/2a, IV/2b, and V/3a, by PCR with genotype-specific primers deduced from the core gene. Sense and antisense primers, specific for each of the five common genotypes, were designed by comparison of 319 core gene sequences from HCV isolates of various...

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Identifiers

PMID: 8880482
DOI: 10.1128/jcm.34.10.2372-2378.1996
PMCID: PMC229272

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