Article
Glutamate transport in Rhodobacter sphaeroides is mediated by a novel binding protein-dependent secondary transport system.
Proceedings of the National Academy of Sciences of the United States of America - 12 Nov 1996
Jacobs M H, van der Heide T, Driessen A J, Konings W N
Abstract excerpt
Growth of a glutamate transport-deficient mutant of Rhodobacter sphaeroides on glutamate as sole carbon and nitrogen source can be restored by the addition of millimolar amounts of Na+. Uptake of glutamate (Kt of 0.2 microM) by the mutant strictly requires Na+ (K(m) of 25 mM) and is inhibited by ionophores that collapse the proton motive force (pmf). The activity is osmotic-shock-sensitive and can be restored in...
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